Journal: Scientific Reports

Article Title: FTY720 in resistant human epidermal growth factor receptor 2-positive breast cancer

doi: 10.1038/s41598-021-04328-y

Figure Lengend Snippet: Xenograft mouse model of HCC1954 cells showing the efficacy of FTY720 with or without trastuzumab. ( a ) Mice bearing HCC1954 xenografts were randomized into four treatment groups (n = 9 for each group) when tumor size reached 150–200 mm 3 . Mice were treated with PBS, trastuzumab, FTY720, or FTY720 plus trastuzumab. Xenografts were harvested 24 h after the last treatment. Each plot indicates the mean increase in tumor volume from the first day of treatment, whereas error bars indicate the standard error of the mean. Differences between the four groups were analyzed using ANOVA with mixed-effect model. Differences in control versus FTY720 and control versus FTY720 plus trastuzumab were statistically significant. P value: * P < 0.05 and ** P < 0.01. ( b ) Tumor tissue sections were evaluated using TUNEL assays (green) and also counterstained with Hoechst 33,342 dye (blue, indicating nuclei). They were then examined using fluorescence microscopy. The top three tissue sections with the most abundant green signals in each group were presented. ( c ) Using the same tissue sections, cell membranes were outlined using HER2-antibody conjugating secondary antibody (red) and then examined using fluorescence microscopy. Bar, 100 μm. F plus T: FTY720 plus trastuzumab.

Article Snippet: After counterstaining with Hoechst 33,342 dye (blue, staining nuclei, Merck KGaA) and HER2 antibody/PE-Texas Red-conjugated secondary antibody (red, plasma membrane, Merck KGaA), the cells were examined using fluorescence microscopy .

Techniques: Control, TUNEL Assay, Fluorescence, Microscopy

Journal: Pharmaceutics

Article Title: Bacterial Cellulose as a Potential Bio-Scaffold for Effective Re-Epithelialization Therapy

doi: 10.3390/pharmaceutics13101592

Figure Lengend Snippet: Immunofluorescence staining of human adipose stem cells cultured on bacterial cellulose scaffold for 14 days. ( a , b ) Octamer-binding protein 4 (OCT-4) and OCT-4/Hoechst overlay expression, respectively. ( c , d ) Nestin and Nestin/Hoechst overlay expression, respectively. ( e , f ) Sex determining region Y-box 9 (SOX-9) and SOX-9/Hoechst overlay expression, respectively (scale bar = 200 µm).

Article Snippet: Hoechst 33,342 (1:5000; AnaSpec, Fremont, CA, USA) was then added to the samples for 15 min for nuclei visualization.

Techniques: Immunofluorescence, Staining, Cell Culture, Binding Assay, Expressing